[Histonet] TESTICULAR BIOSY

Tony Henwood AnthonyH <@t> chw.edu.au
Sun Sep 14 19:28:02 CDT 2003


Beware,
 
Protein picrates are believed to be quite soluble in water. I would
recommend storing in 80% or more ethanol prior to processing, not NBF.
Fixation time 4-8 hours for a small biopsy (up to 4mm in diameter)
 
Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) 
Laboratory Manager 
The Children's Hospital at  Westmead, 
Locked Bag 4001, Westmead, 2145, AUSTRALIA. 
Tel: 612 9845 3306 
Fax: 612 9845 3318 

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-----Original Message-----
From: Travis Troyer [mailto:ttroyer <@t> pcllab.com]
Sent: Friday, 12 September 2003 23:07
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] TESTICULAR BIOSY


Our lab is receiving a testicular biopsy that will be received in Bouin's
Solution.  We routinely place specimans in 10% NBF after grossing.  So, my
question is how long should the biopsy stay in the Bouin's solution for
proper fixation before placing into 10% NBF?
Thanks!



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