[Histonet] Bouin's fixation/removal of picric acid

[Sarah Jones]

I've always used saturated lithium carbonate in 70% ethanol to remove
the picric acid.  I believe it would be faster and more complete than
70% alone.  


Sarah Jones HT(ASCP)
Dept. of Vet. Anatomy & Public Health
Histology Lab
Texas A&M University
College Station, TX 77843-4458
phone: 979-845-3177
fax:  979-458-3499


>>> "peptolab" <peptolab <@t> hamptons.com> 09/13/03 08:32AM >>>
A 3-5 mm testicular biopsy should be fixed in one to two hours. Be sure
to
rinse out as much yellow picric acid as possible (you can use running
water
or 70% alcohol ). The basophilia of chromatin in the stored block will
deteriorate after some time if there is picric acid  residue.

Jeff Silverman HT HTL QIHC (ASCP)
Southside Hospital
Bay Shore NY


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