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<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=250392300-15092003>Beware,</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=250392300-15092003></SPAN></FONT> </DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=250392300-15092003>Protein picrates are believed to be quite soluble in
water. I would recommend storing in 80% or more ethanol prior to processing, not
NBF. Fixation time 4-8 hours for a small biopsy (up to 4mm in
diameter)</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=250392300-15092003></SPAN></FONT> </DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=250392300-15092003>
<P><FONT face=Arial size=2>Tony Henwood JP, B</FONT><FONT face=Arial size=2>A</FONT><FONT face=Arial size=2>ppSc, GradDipSysAnalys, CT(ASC)</FONT>
<BR><FONT face=Arial size=2>Laboratory Manager</FONT> <BR><FONT face=Arial
size=2>The Children's Hospital at Westmead,</FONT> <BR><FONT face=Arial
size=2>Locked Bag 4001, Westmead, 2145, AUSTRALIA.</FONT> <BR><FONT face=Arial
size=2>Tel:</FONT> <FONT face=Arial size=2>61</FONT><FONT face=Arial size=2>2
9845 3306</FONT> <BR><FONT face=Arial size=2>Fax:</FONT> <FONT face=Arial
size=2>61</FONT><FONT face=Arial size=2>2 9845 3318</FONT> </P>
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<BLOCKQUOTE dir=ltr style="MARGIN-RIGHT: 0px">
<DIV class=OutlookMessageHeader dir=ltr align=left><FONT face=Tahoma
size=2>-----Original Message-----<BR><B>From:</B> Travis Troyer
[mailto:ttroyer@pcllab.com]<BR><B>Sent:</B> Friday, 12 September 2003
23:07<BR><B>To:</B> histonet@lists.utsouthwestern.edu<BR><B>Subject:</B> [Histonet] TESTICULAR BIOSY<BR><BR></FONT></DIV>
<DIV><FONT face=Arial size=2>Our lab is receiving a testicular biopsy that
will be received in Bouin's Solution. We routinely place specimans in 10% NBF after grossing. So, my question is how long should
the biopsy stay in the Bouin's solution for proper fixation before placing
into 10% NBF?</FONT></DIV>
<DIV><FONT face=Arial size=2>Thanks!</FONT></DIV></BLOCKQUOTE><CODE><FONT SIZE=3><BR>
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