[Histonet] validating oil red O
Gudrun Lang
g.lang <@t> bigfoot.de
Fri Sep 12 05:13:39 CDT 2003
Don't you use frozen cuts with the fat-staining?
----- Original Message -----
From: Jeremy Browne
To: 'histonet <@t> lists.utsouthwestern.edu'
Sent: Friday, September 12, 2003 3:53 AM
Subject: [Histonet] validating oil red O
Anyone out there with an idea of oil red O staining could be validated?
We need to set up a range of fat concentrations on slides, stain and then check to see that there is a linear response relationship between fat concentration and staining intensity but because fat melts and slides off slides this is not easy. However, I imagine that someone out there is histoworld has solved this problem - perhaps with an intermediate substance to which the fat adheres?
Thanks
Jeremy Browne
Liggins Institute
University of Auckland
NZ (09) 373 7599 extn 86444
j.browne <@t> auckland.ac.nz
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