[Histonet] validating oil red O

[Gudrun Lang]

Don't you use frozen cuts with the fat-staining?

  ----- Original Message ----- 
  From: Jeremy Browne 
  To: 'histonet <@t> lists.utsouthwestern.edu' 
  Sent: Friday, September 12, 2003 3:53 AM
  Subject: [Histonet] validating oil red O


  Anyone out there with an idea of oil red O staining could be validated?

  We need to set up a range of fat concentrations on slides, stain and then check to see that there is a linear response relationship between fat concentration and staining intensity but because fat melts and slides off slides this is not easy.  However, I imagine that someone out there is histoworld has solved this problem - perhaps with an intermediate substance to which the fat adheres?

  Thanks



  Jeremy Browne 

  Liggins Institute

  University of Auckland

  NZ (09) 373 7599 extn 86444

  j.browne <@t> auckland.ac.nz



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