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<DIV><FONT face=Arial size=2>Don't you use frozen cuts with the
fat-staining?</FONT></DIV>
<DIV> </DIV>
<BLOCKQUOTE dir=ltr
style="PADDING-RIGHT: 0px; PADDING-LEFT: 5px; MARGIN-LEFT: 5px; BORDER-LEFT: #000000 2px solid; MARGIN-RIGHT: 0px">
<DIV style="FONT: 10pt arial">----- Original Message ----- </DIV>
<DIV
style="BACKGROUND: #e4e4e4; FONT: 10pt arial; font-color: black"><B>From:</B>
<A title=j.browne@auckland.ac.nz href="mailto:j.browne@auckland.ac.nz">Jeremy
Browne</A> </DIV>
<DIV style="FONT: 10pt arial"><B>To:</B> <A
title=histonet@lists.utsouthwestern.edu
href="mailto:'histonet@lists.utsouthwestern.edu'">'histonet@lists.utsouthwestern.edu'</A>
</DIV>
<DIV style="FONT: 10pt arial"><B>Sent:</B> Friday, September 12, 2003 3:53
AM</DIV>
<DIV style="FONT: 10pt arial"><B>Subject:</B> [Histonet] validating oil red
O</DIV>
<DIV><BR></DIV>
<DIV class=Section1>
<P class=MsoNormal><FONT face=Arial size=2><SPAN lang=EN-AU
style="FONT-SIZE: 10pt; FONT-FAMILY: Arial">Anyone out there with an idea of
oil red O staining could be validated?</SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial size=2><SPAN lang=EN-AU
style="FONT-SIZE: 10pt; FONT-FAMILY: Arial">We need to set up a range of fat
concentrations on slides, stain and then check to see that there is a linear
response relationship between fat concentration and staining intensity but
because fat melts and slides off slides this is not easy. However, I
imagine that someone out there is histoworld has solved this problem - perhaps
with an intermediate substance to which the fat adheres?</SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial size=2><SPAN lang=EN-AU
style="FONT-SIZE: 10pt; FONT-FAMILY: Arial">Thanks</SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial size=2><SPAN lang=EN-AU
style="FONT-SIZE: 10pt; FONT-FAMILY: Arial"></SPAN></FONT> </P>
<P class=MsoNormal><FONT face=Arial size=2><SPAN lang=EN-AU
style="FONT-SIZE: 10pt; FONT-FAMILY: Arial">Jeremy Browne </SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial size=2><SPAN lang=EN-AU
style="FONT-SIZE: 10pt; FONT-FAMILY: Arial">Liggins
Institute</SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial size=2><SPAN lang=EN-AU
style="FONT-SIZE: 10pt; FONT-FAMILY: Arial">University of
Auckland</SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial size=2><SPAN lang=PT-BR
style="FONT-SIZE: 10pt; FONT-FAMILY: Arial">NZ (09) 373 7599 extn
86444</SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial size=2><SPAN lang=EN-AU
style="FONT-SIZE: 10pt; FONT-FAMILY: Arial"><A
href="mailto:j.browne@auckland.ac.nz"><SPAN
lang=PT-BR>j.browne@auckland.ac.nz</SPAN></A></SPAN></FONT></P>
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