[Histonet] validating oil red O

[Jeremy Browne]

Anyone out there with an idea of oil red O staining could be validated?

We need to set up a range of fat concentrations on slides, stain and then
check to see that there is a linear response relationship between fat
concentration and staining intensity but because fat melts and slides off
slides this is not easy.  However, I imagine that someone out there is
histoworld has solved this problem - perhaps with an intermediate substance
to which the fat adheres?

Thanks

 

Jeremy Browne 

Liggins Institute

University of Auckland

NZ (09) 373 7599 extn 86444

 <mailto:j.browne <@t> auckland.ac.nz> j.browne <@t> auckland.ac.nz

 

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