[Histonet] RE: IHC with CD25 on FFPE lymph node

[nick.kirk3 <@t> btopenworld.com]

Philippe

Novocastra produce a CD25 that survives paraffin processing. It's clone number 4C9.
It requires Citrate buffer heat mediated antigen retrieval and its code no is NCL-CD25-305

Online catalogue sheet is at http://www.novocastra.co.uk/data/gfr/cd25-305.pdf

The Canadian distributor is Vector labs


Nick Kirk
Histopathology
Hinchingbrooke Hiospital 
Huntingdon
England

>  from:    "C.M. van der Loos" <c.m.vanderloos <@t> amc.uva.nl>
>  date:    Mon, 08 Sep 2003 10:07:29
>  to:      histonet <@t> lists.utsouthwestern.edu
>  cc:      philippegannon <@t> hotmail.com
>  subject: Re: [Histonet] RE: IHC with CD25 on FFPE lymph node
> 
> Dear Philippe,
> The anti-CD25 Cymbus product CBL151 you indicated is in fact clone 
> Tu69. This clone is unfortunately not working on FFPE sections. In 
> other words, this particular anti-CD25 clone is reacting with an 
> epitope on the CD25 molecule that is not surviving the FFPE procedure. 
> Unfortunately, I am not aware of any anti-CD25 clones working with FFPE 
> sections.
> 
> Chris van der Loos
> Dept. of Cardiovascular Pathology
> Academical Medical Center
> Amsterdam - The Netherlands  
> 
> >From  "Philippe Gannon" <philippegannon <@t> hotmail.com> 
> >Date  Thu, 04 Sep 2003 17:32:25  0000 
> >To  HistoNet <@t> pathology.swmed.edu 
> >Subject  [Histonet] IHC with CD25 on FFPE lymph node 
> >
> >hi,
> >
> >I am having some difficulties with a CD25 staining on formalin-fixed 
> >paraffin embedded lymph node slides. In fact, I am not seeing any 
> >staining at all with the antibody CBL 151 from Cymbus Biotechnology 
> >LTD/Chemicon at concentration up to 1:10 in PBS. 
> >
> >Laboratoire Mes-Masson (Y-4609)
> >Institut du cancer de Montréal, Université de Montréal
> >2099 Alexandre de Sève
> >Montréal, Québec, Canada
> >H2L 2W5
> 
> 
> 
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