[Histonet] RE: IHC with CD25 on FFPE lymph node

[C.M. van der Loos]

Dear Philippe,
The anti-CD25 Cymbus product CBL151 you indicated is in fact clone 
Tu69. This clone is unfortunately not working on FFPE sections. In 
other words, this particular anti-CD25 clone is reacting with an 
epitope on the CD25 molecule that is not surviving the FFPE procedure. 
Unfortunately, I am not aware of any anti-CD25 clones working with FFPE 
sections.

Chris van der Loos
Dept. of Cardiovascular Pathology
Academical Medical Center
Amsterdam - The Netherlands  

>From  "Philippe Gannon" <philippegannon <@t> hotmail.com> 
>Date  Thu, 04 Sep 2003 17:32:25 +0000 
>To  HistoNet <@t> pathology.swmed.edu 
>Subject  [Histonet] IHC with CD25 on FFPE lymph node 
>
>hi,
>
>I am having some difficulties with a CD25 staining on formalin-fixed 
>paraffin embedded lymph node slides. In fact, I am not seeing any 
>staining at all with the antibody CBL 151 from Cymbus Biotechnology 
>LTD/Chemicon at concentration up to 1:10 in PBS. 
>
>Laboratoire Mes-Masson (Y-4609)
>Institut du cancer de Montréal, Université de Montréal
>2099 Alexandre de Sève
>Montréal, Québec, Canada
>H2L 2W5