[Histonet] liquid nitrogen immersion

[Davis, Gareth]

Brandy, 
When we have immersed tissue into Liquid Nitrogen, we have actually made our own cassettes with foil.  We just use regular foil and shape it around a cork or bottom of a small bottle (depending on size needed), leaving extra to fold over the frozen block - when finished freezing.  Put the freezing medium and tissue in foil cassette and immerse in LN until just about completely frozen, =small bubble in center results.  Then remove and store as in -80. 
Hope this helps.
Gareth
 
Ms. Gareth B. Davis
Research Assistant II
Neuro-magnetics Division of 
the Department of Neurology
Vanderbilt University Medical Center
615-936-3318
 
 

	-----Original Message----- 
	From: Brandy Baca [mailto:BRBaca <@t> salud.unm.edu] 
	Sent: Thu 10/30/2003 3:51 PM 
	To: histonet <@t> lists.utsouthwestern.edu 
	Cc: 
	Subject: [Histonet] liquid nitrogen immersion
	
	

	I am new to the listserve and have a question regarding immersion of
	cassettes and vials in liquid nitrogen. I am going to be working with a
	developing tissue bank and wondered if anyone knows what cassettes and
	vials can withstand immersion in liquid nitrogen for quick-freezing. I
	have spoke with Sakura and they have no evidence that their
	uni-cassettes can withstand immersion. As for vials, many don't
	reccomend immersion but some require a tube in order to accomodate this
	process. If anyone can suggest a manufacturer and type, it would be
	greatly appreciated. I would like to thank everyone in advance for their
	responses.
	
	
	
	Brandy Baca
	Dept of Pathology
	CRTC B-93
	505.272.1127 phone
	505.272.1370 fax
	
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