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<DIV>Brandy, </DIV>
<DIV>When we have immersed tissue into Liquid Nitrogen, we have actually made
our own cassettes with foil. We just use regular foil and shape it around
a cork or bottom of a small bottle (depending on size needed), leaving extra to
fold over the frozen block - when finished freezing. Put the freezing
medium and tissue in foil cassette and immerse in LN until just about completely
frozen, =small bubble in center results. Then remove and store as in -80.
</DIV>
<DIV>Hope this helps.</DIV>
<DIV>Gareth</DIV>
<DIV> </DIV>
<DIV><FONT face=System color=#800080 size=2>Ms. Gareth B. Davis</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2><STRONG>Research Assistant
II</STRONG></FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>Neuro-magnetics Division of
</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>the Department of
Neurology</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>Vanderbilt University Medical
Center</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>615-936-3318</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2></FONT> </DIV>
<DIV> </DIV>
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<DIV><FONT size=2>-----Original Message----- <BR><B>From:</B> Brandy Baca
[mailto:BRBaca@salud.unm.edu] <BR><B>Sent:</B> Thu 10/30/2003 3:51 PM
<BR><B>To:</B> histonet@lists.utsouthwestern.edu <BR><B>Cc:</B>
<BR><B>Subject:</B> [Histonet] liquid nitrogen immersion<BR><BR></FONT></DIV>
<P><FONT size=2>I am new to the listserve and have a question regarding
immersion of<BR>cassettes and vials in liquid nitrogen. I am going to be
working with a<BR>developing tissue bank and wondered if anyone knows what
cassettes and<BR>vials can withstand immersion in liquid nitrogen for
quick-freezing. I<BR>have spoke with Sakura and they have no evidence that
their<BR>uni-cassettes can withstand immersion. As for vials, many
don't<BR>reccomend immersion but some require a tube in order to accomodate
this<BR>process. If anyone can suggest a manufacturer and type, it would
be<BR>greatly appreciated. I would like to thank everyone in advance for
their<BR>responses.<BR><BR><BR><BR>Brandy Baca<BR>Dept of Pathology<BR>CRTC
B-93<BR>505.272.1127 phone<BR>505.272.1370
fax<BR><BR>_______________________________________________<BR>Histonet mailing
list<BR>Histonet@lists.utsouthwestern.edu<BR><A
href="http://lists.utsouthwestern.edu/mailman/listinfo/histonet">http://lists.utsouthwestern.edu/mailman/listinfo/histonet</A><BR></FONT></P></BLOCKQUOTE>
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