[Histonet] Grocott's stain for fungi

[Kathleen Roberts]

Yes, that is what I am trying to dissolve-I just wrote "methenamine" 
because it's an alternative name for hexamethylenetetramine and it's 
easier to type. :o) Plus, that's what the AFIP manual calls it. 

Thanks!
Kathleen
Rutgers University

Jackie.O'Connor <@t> abbott.com wrote:

>
> Make sure you are using the correct chemical, it should be 
> Hexamethylenetetramine (methenamine) (C6H12N4).  It should dissolve 
> readily in H20.
>
> Jacqueline M. O'Connor HT(ASCP)
> Abbott Laboratories
> Global Pharmaceutical Research and Development
> Discovery Chemotheraputics
>
>
>
>
> 	Kathleen Roberts <kgrobert <@t> rci.rutgers.edu>
> Sent by: histonet-admin <@t> lists.utsouthwestern.edu
>
> 10/28/2003 09:51 AM
>
> 	       
>         To:        Histonet <histonet <@t> pathology.swmed.edu>
>         cc:        
>         Subject:        [Histonet] Grocott's stain for fungi
>
>
>
>
> Hi all-
>
> I'm making up the various stock & working solutions for Grocott's stain,
> and I am having a heck of a time getting the 3% methenamine to go into
> solution.  (Not methenamine silver, just plain old 3% methenamine in
> distilled water.)  I know that you have to acid-clean the glassware for
> the methenamine silver & silver nitrate solutions, but do I have to do
> it for 3% methenamine?  I tried heating it, but that did not make a bit
> of difference. AFIP manual  has no other instructions other than "3 g
> methenamine in 100 ml distilled water".
>
> Is there a shelf life for methenamine?  The bottle we have is pretty
> old, but I am not sure if that would be a factor.
>
> I intend to try acid-cleaning a bottle for the 3% methenamine this
> afternoon, but I wanted to see if anybody had any other suggestions to
> try as well.
>
> Thanks in advance-
> Kathleen Roberts
> Principal Lab Technician
> Neurotoxicology Labs
> Dept of Pharmacology & Toxicology
> Ernest Mario School of Pharmacy
> Rutgers University
>
>
>
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