<!DOCTYPE html PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN">
<html>
<head>
<meta http-equiv="Content-Type" content="text/html;charset=ISO-8859-1">
<title></title>
</head>
<body text="#000000" bgcolor="#ffffff">
Yes, that is what I am trying to dissolve-I just wrote "methenamine"
because it's an alternative name for hexamethylenetetramine and it's
easier to type. :o) Plus, that's what the AFIP manual calls it. <br>
<br>
Thanks!<br>
Kathleen<br>
Rutgers University<br>
<br>
Jackie.O'<a class="moz-txt-link-abbreviated" href="mailto:Connor@abbott.com">Connor@abbott.com</a> wrote:<br>
<blockquote type="cite"
cite="midOFBD37036C.B9218C8C-ON86256DCD.005D5082@northamerica.intra.abbott.com"><br>
<font size="2" face="Arial">Make sure you are using the correct
chemical, it should be Hexamethylenetetramine (methenamine) (C6H12N4).
It should dissolve readily in H20.</font>
<br>
<br>
<font size="2" face="Arial">Jacqueline M. O'Connor HT(ASCP)<br>
Abbott Laboratories<br>
Global Pharmaceutical Research and Development<br>
Discovery Chemotheraputics<br>
</font>
<br>
<br>
<br>
<table width="100%">
<tbody>
<tr valign="top">
<td>
<br>
</td>
<td><font size="1" face="sans-serif"><b>Kathleen Roberts
<a class="moz-txt-link-rfc2396E" href="mailto:kgrobert@rci.rutgers.edu"><kgrobert@rci.rutgers.edu></a></b></font>
<br>
<font size="1" face="sans-serif">Sent by:
<a class="moz-txt-link-abbreviated" href="mailto:histonet-admin@lists.utsouthwestern.edu">histonet-admin@lists.utsouthwestern.edu</a></font>
<p><font size="1" face="sans-serif">10/28/2003 09:51 AM</font>
<br>
</p>
</td>
<td><font size="1" face="Arial"> </font>
<br>
<font size="1" face="sans-serif"> To: Histonet
<a class="moz-txt-link-rfc2396E" href="mailto:histonet@pathology.swmed.edu"><histonet@pathology.swmed.edu></a></font>
<br>
<font size="1" face="sans-serif"> cc: </font>
<br>
<font size="1" face="sans-serif"> Subject:
[Histonet] Grocott's stain for fungi</font></td>
</tr>
</tbody>
</table>
<br>
<br>
<br>
<font size="2" face="Courier New">Hi all-<br>
<br>
I'm making up the various stock & working solutions for Grocott's
stain, <br>
and I am having a heck of a time getting the 3% methenamine to go into <br>
solution. (Not methenamine silver, just plain old 3% methenamine in <br>
distilled water.) I know that you have to acid-clean the glassware for
<br>
the methenamine silver & silver nitrate solutions, but do I have to
do <br>
it for 3% methenamine? I tried heating it, but that did not make a bit
<br>
of difference. AFIP manual has no other instructions other than "3 g <br>
methenamine in 100 ml distilled water".<br>
<br>
Is there a shelf life for methenamine? The bottle we have is pretty <br>
old, but I am not sure if that would be a factor.<br>
<br>
I intend to try acid-cleaning a bottle for the 3% methenamine this <br>
afternoon, but I wanted to see if anybody had any other suggestions to <br>
try as well.<br>
<br>
Thanks in advance-<br>
Kathleen Roberts<br>
Principal Lab Technician<br>
Neurotoxicology Labs<br>
Dept of Pharmacology & Toxicology<br>
Ernest Mario School of Pharmacy<br>
Rutgers University<br>
<br>
<br>
<br>
_______________________________________________<br>
Histonet mailing list<br>
<a class="moz-txt-link-abbreviated" href="mailto:Histonet@lists.utsouthwestern.edu">Histonet@lists.utsouthwestern.edu</a><br>
<a class="moz-txt-link-freetext" href="http://lists.utsouthwestern.edu/mailman/listinfo/histonet">http://lists.utsouthwestern.edu/mailman/listinfo/histonet</a><br>
</font>
<br>
<br>
</blockquote>
</body>
</html>