[Histonet] Grocott's stain for fungi

[Jackie.O'Connor <@t> abbott.com]

Make sure you are using the correct chemical, it should be 
Hexamethylenetetramine (methenamine) (C6H12N4).  It should dissolve 
readily in H20.

Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotheraputics





Kathleen Roberts <kgrobert <@t> rci.rutgers.edu>
Sent by: histonet-admin <@t> lists.utsouthwestern.edu
10/28/2003 09:51 AM

 
        To:     Histonet <histonet <@t> pathology.swmed.edu>
        cc: 
        Subject:        [Histonet] Grocott's stain for fungi


Hi all-

I'm making up the various stock & working solutions for Grocott's stain, 
and I am having a heck of a time getting the 3% methenamine to go into 
solution.  (Not methenamine silver, just plain old 3% methenamine in 
distilled water.)  I know that you have to acid-clean the glassware for 
the methenamine silver & silver nitrate solutions, but do I have to do 
it for 3% methenamine?  I tried heating it, but that did not make a bit 
of difference. AFIP manual  has no other instructions other than "3 g 
methenamine in 100 ml distilled water".

 Is there a shelf life for methenamine?  The bottle we have is pretty 
old, but I am not sure if that would be a factor.

I intend to try acid-cleaning a bottle for the 3% methenamine this 
afternoon, but I wanted to see if anybody had any other suggestions to 
try as well.

Thanks in advance-
Kathleen Roberts
Principal Lab Technician
Neurotoxicology Labs
Dept of Pharmacology & Toxicology
Ernest Mario School of Pharmacy
Rutgers University



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