<br><font size=2 face="Arial">Make sure you are using the correct chemical, it should be Hexamethylenetetramine (methenamine) (C6H12N4). It should dissolve readily in H20.</font>
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<br><font size=2 face="Arial">Jacqueline M. O'Connor HT(ASCP)<br>
Abbott Laboratories<br>
Global Pharmaceutical Research and Development<br>
Discovery Chemotheraputics<br>
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<td><font size=1 face="sans-serif"><b>Kathleen Roberts <kgrobert@rci.rutgers.edu></b></font>
<br><font size=1 face="sans-serif">Sent by: histonet-admin@lists.utsouthwestern.edu</font>
<p><font size=1 face="sans-serif">10/28/2003 09:51 AM</font>
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<br><font size=1 face="sans-serif"> To: Histonet <histonet@pathology.swmed.edu></font>
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<br><font size=1 face="sans-serif"> Subject: [Histonet] Grocott's stain for fungi</font></table>
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<br><font size=2 face="Courier New">Hi all-<br>
<br>
I'm making up the various stock & working solutions for Grocott's stain, <br>
and I am having a heck of a time getting the 3% methenamine to go into <br>
solution. (Not methenamine silver, just plain old 3% methenamine in <br>
distilled water.) I know that you have to acid-clean the glassware for <br>
the methenamine silver & silver nitrate solutions, but do I have to do <br>
it for 3% methenamine? I tried heating it, but that did not make a bit <br>
of difference. AFIP manual has no other instructions other than "3 g <br>
methenamine in 100 ml distilled water".<br>
<br>
Is there a shelf life for methenamine? The bottle we have is pretty <br>
old, but I am not sure if that would be a factor.<br>
<br>
I intend to try acid-cleaning a bottle for the 3% methenamine this <br>
afternoon, but I wanted to see if anybody had any other suggestions to <br>
try as well.<br>
<br>
Thanks in advance-<br>
Kathleen Roberts<br>
Principal Lab Technician<br>
Neurotoxicology Labs<br>
Dept of Pharmacology & Toxicology<br>
Ernest Mario School of Pharmacy<br>
Rutgers University<br>
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