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<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=515223901-10102003>You
could try secondary fixing the tissue in chromate solution to get the Chromaffin
reaction, it usually works.</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=515223901-10102003></SPAN></FONT> </DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=515223901-10102003>Nick
Kirk</SPAN></FONT></DIV>
<BLOCKQUOTE dir=ltr style="MARGIN-RIGHT: 0px">
<DIV class=OutlookMessageHeader dir=ltr align=left><FONT face=Tahoma
size=2>-----Original Message-----<BR><B>From:</B>
histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-admin@lists.utsouthwestern.edu]<B>On Behalf Of </B>Geoff
McAuliffe<BR><B>Sent:</B> 09 October 2003 22:15<BR><B>To:</B> Nick
Kirk<BR><B>Cc:</B> Histonet<BR><B>Subject:</B> Re: [Histonet] Need silver
stain for granules in adrenals<BR><BR></FONT></DIV>I don't think you can get a
good chromaffin reaction on material already fixed with formalin.
Fontana-Masson should be fine. John Kiernan published a chromate-dichromate
fix for chromaffin tissues, it may be in his book, along with other
recommendations.<BR><BR>Geoff<BR><BR>Nick Kirk wrote:<BR>
<BLOCKQUOTE cite=midDHEJLPLDFDGJCMMAGCIGCEEMCBAA.nick.kirk3@btopenworld.com
type="cite">
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<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=046044117-09102003>What you are trying to demonstrate are Chromaffin
cells of the adrenal glands so a Masson-Fontana should work, if not try a
Schmorl or carry out the Chromaffin reaction</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=046044117-09102003>If
you use Iodate oxidation you can distinguish between Adrenaline and
noradrenalin fairly easily as iodates oxidise noradrenaline much quicker
than they do adrenaline.</SPAN></FONT></DIV>
<DIV> </DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=046044117-09102003>Nick Kirk</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=046044117-09102003>Histopathology</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=046044117-09102003>Hinchingbrooke Hospital</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=046044117-09102003>Huntingdon</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=046044117-09102003>England</SPAN></FONT></DIV>
<BLOCKQUOTE dir=ltr style="MARGIN-RIGHT: 0px">
<DIV class=OutlookMessageHeader dir=ltr align=left><FONT face=Tahoma
size=2>-----Original Message-----<BR><B>From:</B> <A
class=moz-txt-link-abbreviated
href="mailto:histonet-admin@lists.utsouthwestern.edu">histonet-admin@lists.utsouthwestern.edu</A>
[<A class=moz-txt-link-freetext
href="mailto:histonet-admin@lists.utsouthwestern.edu">mailto:histonet-admin@lists.utsouthwestern.edu</A>]<B>On
Behalf Of </B>Nancy Maronto<BR><B>Sent:</B> 09 October 2003
16:05<BR><B>To:</B> <A class=moz-txt-link-abbreviated
href="mailto:histonet@lists.utsouthwestern.edu">histonet@lists.utsouthwestern.edu</A><BR><B>Subject:</B>
[Histonet] Need silver stain for granules in adrenals<BR><BR></FONT></DIV>
<DIV>Hi,</DIV>
<DIV>Could anyone suggest a stain for noradrenalin and adrenalin granules
in paraffin embedded adrenals. We were asked to do a gremilius stain
and it did not stain the granules. Are these slides lost or can we
re stain them? We usually do a recut of the block, but it is asked
if we could use the same tissue slide. The stain actually worked, but not
for the granules.</DIV>
<DIV> </DIV>
<DIV>From what we read a Fontana-masson should work. There probably
is a better stain our there for this specific target. Can anyone
with some experience with staining these granules share their staining
protocol.</DIV>
<DIV> </DIV>
<DIV>At this time the request is not for
immuno staining. </DIV>
<DIV> </DIV>
<DIV>Thanks for your help.</DIV>
<DIV> </DIV>
<DIV>Nancy Maronto</DIV></BLOCKQUOTE></BLOCKQUOTE><BR><PRE class=moz-signature cols="72">--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
<A class=moz-txt-link-abbreviated href="mailto:mcauliff@umdnj.edu">mcauliff@umdnj.edu</A>
**********************************************</PRE></BLOCKQUOTE></BODY></HTML>