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<TITLE>RE: [Histonet] warthin starry</TITLE>
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<P><FONT COLOR="#0000FF" SIZE=2 FACE="Arial">Hi Celia,</FONT>
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<P><FONT COLOR="#0000FF" SIZE=2 FACE="Arial">We also had problems with gastric bx's and it wasn't a consistent problem. I retrieved them and then stained them H&E and they were much better. It did not seem to matter what you retrieved them with either....very weird. We think it is something with the fixation. I had a problem with bone marrows and it turned out to be, the decal was messed up, but I retrieved them and they stained better. Go figure.... but it worked. My Pathologist also heard about this at a conference he went to. They did not know why it worked either. We do the microwave Steiner now and it works much better. Try the retrieval...good luck.</FONT></P>
<P><FONT COLOR="#0000FF" SIZE=2 FACE="Arial">Carole Fields</FONT>
<BR><FONT COLOR="#0000FF" SIZE=2 FACE="Arial">Lexington Medical Center</FONT>
<BR><FONT COLOR="#0000FF" SIZE=2 FACE="Arial">W.Columbia, SC </FONT>
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<P><FONT SIZE=1 FACE="Arial">-----Original Message-----</FONT>
<BR><B><FONT SIZE=1 FACE="Arial">From: </FONT></B> <FONT SIZE=1 FACE="Arial">celia [SMTP:cfarm@sio.midco.net]</FONT>
<BR><B><FONT SIZE=1 FACE="Arial">Sent: </FONT></B> <FONT SIZE=1 FACE="Arial">Wednesday, October 08, 2003 6:23 PM</FONT>
<BR><B><FONT SIZE=1 FACE="Arial">To: </FONT></B> <FONT SIZE=1 FACE="Arial">histonet@lists.utsouthwestern.edu</FONT>
<BR><B><FONT SIZE=1 FACE="Arial">Subject: </FONT></B> <FONT SIZE=1 FACE="Arial">[Histonet] warthin starry</FONT>
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<P><FONT FACE="Arial">Hello Everyone,</FONT>
<BR><FONT FACE="Arial"> </FONT>
<BR><FONT SIZE=2 FACE="Arial">I have put this question out once before and I didn't get much for a response so I thought I would try putting it out there one more time. I have an ongoing problem with my Warthin-Starry stain. The peripheral edges of some of the gastric biopsies are not staining. I am also experiencing this same non-staining with the Steiner stain on the Ventana stainer. I have tried reducing the temp on the processor down from 60c to 58c thinking they might be "cooking" on the processor (the H&E's look fine) as well as checking the ph of my solutions. This did not work. Could this be a handling problem prior to fixation in 10% NBF? I am at my wits end. Any suggestions will be greatly appreciated.</FONT></P>
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<BR><FONT SIZE=2 FACE="Arial">Thanks in advance.</FONT>
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<BR><FONT SIZE=2 FACE="Arial">Celia, HT</FONT>
<BR><FONT SIZE=2 FACE="Arial">SVH, Sioux Falls, SD</FONT>
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