[Histonet] RE: mast cells

[C.M. van der Loos]

Hi Noreen,
Apart from the histology solutions there is a very good IHC staining 
for mast cells too. We have been successful using a directly alk. 
phosphatase labeled anti-tryptase antibody from Chemicon (MAB1222A). 
Tryptase is an enzyme present in mast cells only. Beautiful staining on 
both cryostat (1:500, overnight 4C) or FFPE sections (HIER with 
citrate6.0, 1:500 overnight 4C). For alk. phosphatase chromogen use 
either New Fuchsin (Dako K0596), Permanent Red (Dako K0695) or Vector 
Red (SK-5100). 

Chris van der Loos
Dept. of Cardiovascular Pathology
Academical Medical Center
Amsterdam - The Netherlands

----- Original Message ----- 
>From  "Noreen Gilman" <Ngilman <@t> nbhd.org> 
Date  Wed, 01 Oct 2003 15:00:20 -0400 
To  <histonet <@t> lists.utsouthwestern.edu> 
Subject  [Histonet] mast cells 

Can anyone recommend a stain for mast cells? The one we do just doesn't 
seem to be working as well as we'd like it to.
thanks
noreen
 
Noreen Gilman, BS, HT (ASCP) QIHC
Histopathology Supervisor
Broward General Medical Center
Ft. Lauderdale, FL 33316