[Histonet] Re: RE: mast cells

[C.M. van der Loos]

Hi Carol,

I just want to let you know this!

Thanks for the info you had send me
Chris

----- Original Message -----
From: "C.M. van der Loos" <c.m.vanderloos <@t> amc.uva.nl>
Date: Friday, October 3, 2003 10:56 am
Subject: RE: mast cells 

> Hi Noreen,
> Apart from the histology solutions there is a very good IHC 
> staining for mast cells too. We have been successful using a directly 
> alk. phosphatase labeled anti-tryptase antibody from Chemicon 
> (MAB1222A). 
> Tryptase is an enzyme present in mast cells only. Beautiful staining 
> on both cryostat (1:500, overnight 4C) or FFPE sections (HIER with 
> citrate6.0, 1:500 overnight 4C). For alk. phosphatase chromogen 
> use either New Fuchsin (Dako K0596), Permanent Red (Dako K0695) or 
> Vector Red (SK-5100). 
> 
> Chris van der Loos
> Dept. of Cardiovascular Pathology
> Academical Medical Center
> Amsterdam - The Netherlands
> 
> ----- Original Message ----- 
> From  "Noreen Gilman" <Ngilman <@t> nbhd.org> 
> Date  Wed, 01 Oct 2003 15:00:20 -0400 
> To  <histonet <@t> lists.utsouthwestern.edu> 
> Subject  [Histonet] mast cells 
> 
> Can anyone recommend a stain for mast cells? The one we do just 
> doesn't 
> seem to be working as well as we'd like it to.
> thanks
> noreen
> 
> Noreen Gilman, BS, HT (ASCP) QIHC
> Histopathology Supervisor
> Broward General Medical Center
> Ft. Lauderdale, FL 33316
> 
> 
>