[Histonet] Double immunostaining

[Tan, MinHan]

Thanks all for the very considered answers to my last question. 
 
1. I would like to enquire - I am planning on using rhodamine and FITC
to double stain my tissue slides with anti-p21 and anti-p27.
Unfortunately, both are nuclear proteins, and I have heard horror
stories from a couple of colleagues on the difficulties of staining the
same structure with different dyes. According to them, usually only one
dye is predominant. Does anyone have any experiences attempting to stain
the nucleus with two different dyes, or would the recommended approach
be to stain two adjacent sections?
 
2. I have noted an apparent artefact during my ABC-DAB
immunohistochemical staining of tissue. The tissue border stains heavily
(an obvious artefact), but just below the border, there is a rim which
completely doesn't stain at all, in stark contrast to the centre of the
section. Has anyone else experienced this? 

Thank you!
 
Min-Han Tan
 

This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information.  Any unauthorized review, use, disclosure or distribution is prohibited.  If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message.  Thank you.
-------------- next part --------------
An HTML attachment was scrubbed...
URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031126/980b17f4/attachment.htm