[Histonet] In situ hybridization

[Jimmy Lao]

Susan Wells,
This is exactly a set of four books.
ISBN: 047150338x
I searched half.com, there are new and used book of
this item there. new one cost $647.30, the used one
$100 but only volumn 1&2.
I couldn't recommend our Lab wibsite due to under
construction. but for in situ protocol, you can  
visit:http://www.rodentia.com/wmc/docs/Big_In_Situ.html
I found it is similar to the one I used.
Hope this help.
Zhimin Lao

--- Susan Q Wells <susan.wells <@t> bms.com> wrote:
> A few days ago I saw a post from you regarding a
> book I'm having
> trouble finding.
> Current protocols in molecular biology
> volume2.edited by
> Frederick Ausubel et al.Printed by John Wiley&sons
> inc.Could you
> check the book again.I cannot find anything on this
> book and I am
> intersetd in ordering it.Perhaps Frederick Ausubel
> wrote it or I
> have the title wrong?
> Thanks,
> Susan Wells HT,QIHC(ASCP)
> 
> Jimmy Lao wrote:
> 
> > Hi, Carlene Zindi,
> > It seems you are going to do In situ hbridization
> on
> > slides. I would like to answer your question based
> on
> > this point with my experience.
> > 1. We used dig conjugated RNA probes like fgf8,
> otx2,
> > Shh and so on for mouse embryo and the brain,
> limbs
> > from bigger than E12.5 mouse.  They work fine.
> > 2. The fix method is that 4% paraformaldehyde in
> PBS
> > at 4 C overnight with gentl nutation. Wash in PBS
> 5
> > min at room temperature for two times. embed in
> OCT
> > crystat section 10-14um thickness.
> > 3. About 1Kb anti-sense probe is reasonable, and
> it is
> > better using 3' UTR(untranslation region) due to
> not
> > much conserved.
> > Hope this help.
> > Zhimin Lao
> >
> > --- "Carlene L. Zindl" <clzindl <@t> artsci.wustl.edu>
> > wrote:
> > > Hello,
> > >
> > > Does anyone have experience with non-radioactive
> in
> > > situ hybridization of
> > > frozen mouse tissue??  If so, I would appreciate
> > > advice on details such
> > > as: 1) is biotin or dig better? 2) what is the
> best
> > > way to fix this
> > > tissue?  3) what is the optimal size RNA probe?
> > >
> > > Thank you for any pertinent information.
> > > Sincerely,
> > > Carlene Zindl
> > > University of Alabama at Birmingham
> > > clzindl <@t> artsci.wustl.edu -OR-  clzindl <@t> uab.edu
> > >
> > >
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