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<DIV><SPAN class=208490417-26112003><FONT face=Arial size=2>Thanks all for the
very considered answers to my last question. </FONT></SPAN></DIV>
<DIV><SPAN class=208490417-26112003><FONT face=Arial
size=2></FONT></SPAN> </DIV>
<DIV><FONT face=Arial><FONT size=2><SPAN class=208490417-26112003>1. I would
like to enquire - I am planning on using rhodamine and FITC to double stain my
tissue slides with anti-p21 and anti-p27. Unfortunately, both are nuclear
proteins, and </SPAN></FONT></FONT><SPAN class=208490417-26112003><FONT
face=Arial size=2>I have heard horror stories from a couple of colleagues on the
difficulties of staining the same structure with different dyes. According to
them, usually only one dye is predominant. </FONT></SPAN><SPAN
class=208490417-26112003><FONT face=Arial size=2>Does anyone have any
experiences attempting to stain the nucleus with two different dyes, or would
the recommended approach be to stain two adjacent sections?</FONT></SPAN></DIV>
<DIV><SPAN class=208490417-26112003><FONT face=Arial
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=208490417-26112003><FONT face=Arial size=2>2. I have noted an
apparent artefact during my ABC-DAB immunohistochemical staining of tissue. The
tissue border stains heavily (an obvious artefact), but just below the border,
there is a rim which completely doesn't stain at all, in stark contrast to the
centre of the section. Has anyone else experienced this?
<BR></FONT></SPAN></DIV>
<DIV><SPAN class=208490417-26112003><FONT face=Arial size=2>Thank
you!</FONT></SPAN></DIV>
<DIV><SPAN class=208490417-26112003><FONT face=Arial
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=208490417-26112003><FONT face=Arial size=2>Min-Han
Tan</DIV></FONT></SPAN>
<DIV><SPAN class=208490417-26112003><FONT face=Arial
size=2></FONT></SPAN> </DIV>
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