Fwd: [Histonet] Eosinophil stains for mouse tissue

[Ian Montgomery]

Judi,
         Recently this has been the bane of my life, eosinophils in mouse 
tissue. Name it and I've tried it. Carbol chromotrope, lovely on other 
species. Various combinations of eosin, strong, weak, long staining, short 
staining. Luna's eosinophil granule stain, biebrich scarlet. For me, the 
best technique by far for mouse tissue is, Sirius Red (Llewellyn,B.D. 1970. 
J. Med. Technol. 27. 308-309). Works a treat every time. All it needs is a 
light Haematoxylin and you have lovely stain. I would try immuno but I'm 
having a bit of bother finding an antibody, anybody suggest a source?
Ian.



>Hi everyone,
>
>We are looking for a stain which will emphasize the eosinophils in mouse 
>heart and lung tissue.  Any help is greatly appreciated.
>
>Thanks,
>
>Judi Ford
>Jackson Laboratory
>Bar Harbor, ME
>
>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Dr. Ian Montgomery,
Histotechnology,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883
e-mail: ian.montgomery <@t> bio.gla.ac.uk 
-------------- next part --------------
An HTML attachment was scrubbed...
URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031118/369ef34b/attachment.htm