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<font size=3>Judi,<br>
<x-tab> </x-tab>Recently
this has been the bane of my life, eosinophils in mouse tissue. Name it
and I've tried it. Carbol chromotrope, lovely on other species. Various
combinations of eosin, strong, weak, long staining, short staining.
Luna's eosinophil granule stain, biebrich scarlet. For me, the best
technique by far for mouse tissue is, Sirius Red (Llewellyn,B.D. 1970. J.
Med. Technol. <b>27.</b> 308-309). Works a treat every time. All it needs
is a light Haematoxylin and you have lovely stain. I would try immuno but
I'm having a bit of bother finding an antibody, anybody suggest a
source?<br>
Ian. <br><br>
<br><br>
<blockquote type=cite class=cite cite>Hi everyone,<br><br>
We are looking for a stain which will emphasize the eosinophils in mouse
heart and lung tissue. Any help is greatly appreciated.
<br><br>
Thanks,<br><br>
Judi Ford<br>
Jackson Laboratory<br>
Bar Harbor, ME<br><br>
<br><br>
<br>
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<x-sigsep><p></x-sigsep>
<font size=3 color="#0000FF">Dr. Ian Montgomery,<br>
Histotechnology,<br>
Graham Kerr Building,<br>
Institute of Biomedical & Life Sciences,<br>
University of Glasgow,<br>
Glasgow,<br>
G12 8QQ.<br>
Tel: 0141 339 8855 <br>
Office: 4652<br>
Lab: 6644.<br>
Pager: 07625 702883<br>
e-mail: ian.montgomery@bio.gla.ac.uk</font></body>
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