[Histonet] DBA staining for parietal cells

[Barry R Rittman]

DBA (Dolichos biflorus) binds to several types of N-acetyl glucosamines.
I would go to two sources.
First for a history of lectins "The Lectins. Properties, Functions and
Applications in Biology and Medicine" by Liener, Sharon and Goldstein.
Academic Press. Published in 1986. Although this is an older text it is
a excellent resource for information on all the lectins known up to that
time.
Another great sources is Vector Laboratories, I think still at
Burlingame, CA.. They have a booklet that provides a lot of information
re lectins. They do sell lectins and lectin antibodies and all those I
have purchased from them have been outstanding.
 
Some cautions
If you are going to use a lectin bound to a peroxidase then these do
often have a limited life span.
For DBA and many other lectins there are a number of isolectins with
slightly different binding properties and specificities. 
Few lectins bind to only one carbohydrate moiety and therefore the
carbohydrate you use for negative controls must take this into account.
Negative controls using specific (and highly purified carbohydrates) are
critical in evaluating lectin binding.
 
Barry
713-500-4134
6516 MD Anderson Blvd.
Houston, TX. 77030
 

	-----Original Message-----
	From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu] On Behalf Of Manuelle
De Bock
	Sent: Friday, November 14, 2003 2:15 AM
	To: histonet <@t> lists.utsouthwestern.edu
	Subject: [Histonet] DBA staining for parietal cells
	
	
	
	
	Hello everybody,
	 
	First of all I want to thank you all for helping me with my T
and B cell staining problem. I got many replies and am working out some
new protocols now. Thanks!
	I also have a new question, has someone already worked with the
lectin DBA (horse gram) to color parietal cells? 
	Could you give me some more information about that (what are the
bindingplaces of this lectin on the parietal cell?) I have not worked
with lectins yet.
	 
	Thanks!
	 
	Manuelle
	 
	Manuelle De Bock, dierenarts (DVM)
	Laboratory of Veterinary Pathology 
	Department of Pathology, Bacteriology and Avian Diseases
	Faculty of Veterinary Medicine
	Ghent University
	Salisburylaan 133
	B9820 Merelbeke - Belgium
	Tel 0032(0)9 264 7745
	Fax 0032(0)9 264 7789

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