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<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=833261116-14112003>DBA
(Dolichos biflorus) binds to several types of N-acetyl glucosamines. I would go
to two sources.</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=833261116-14112003>First
for a history of lectins "The Lectins. Properties, Functions and Applications in
Biology and Medicine" by Liener, Sharon and Goldstein. Academic Press.
Published in 1986. Although this is an older text it is a excellent resource for
information on all the lectins known up to that time.</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=833261116-14112003>Another great sources is Vector Laboratories, I think
still at Burlingame, CA.. They have a booklet that provides a lot of information
re lectins. They do sell lectins and lectin antibodies and all those I have
purchased from them have been outstanding.</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=833261116-14112003></SPAN></FONT> </DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=833261116-14112003>Some
cautions</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=833261116-14112003>If you
are going to use a lectin bound to a peroxidase then these do often have a
limited life span.</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=833261116-14112003>For
DBA and many other lectins there are a number of isolectins with slightly
different binding properties and specificities. </SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=833261116-14112003>Few
lectins bind to only one carbohydrate moiety and therefore the carbohydrate you
use for negative controls must take this into account.</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=833261116-14112003>Negative controls using specific (and highly purified
carbohydrates) are critical in evaluating lectin binding.</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=833261116-14112003></SPAN></FONT> </DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=833261116-14112003>Barry</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=833261116-14112003>713-500-4134</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=833261116-14112003>6516
MD Anderson Blvd.</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=833261116-14112003>Houston, TX. 77030</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=833261116-14112003></SPAN></FONT> </DIV>
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<DIV></DIV>
<DIV class=OutlookMessageHeader lang=en-us dir=ltr align=left><FONT
face=Tahoma size=2>-----Original Message-----<BR><B>From:</B>
histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-admin@lists.utsouthwestern.edu] <B>On Behalf Of </B>Manuelle
De Bock<BR><B>Sent:</B> Friday, November 14, 2003 2:15 AM<BR><B>To:</B>
histonet@lists.utsouthwestern.edu<BR><B>Subject:</B> [Histonet] DBA staining
for parietal cells<BR><BR></FONT></DIV>
<DIV><FONT face=Arial size=2>
<DIV><FONT face=Arial size=2>
<DIV><FONT face=Arial size=2>Hello everybody,</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>First of all I want to thank you all for helping
me with my T and B cell staining problem. I got many replies and am working
out some new protocols now. Thanks!</FONT></DIV>
<DIV><FONT face=Arial size=2>I also have a new question, has someone
already worked with the lectin DBA (horse gram) to color parietal cells?
</FONT></DIV>
<DIV><FONT face=Arial size=2>Could you give me some more information about
that (what are the bindingplaces of this lectin on the parietal cell?) I have
not worked with lectins yet.</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Thanks!</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Manuelle</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV></FONT></DIV></FONT></DIV>
<DIV><FONT face=Arial size=2>Manuelle De Bock, dierenarts (DVM)<BR>Laboratory
of Veterinary Pathology <BR>Department of Pathology, Bacteriology and Avian
Diseases<BR>Faculty of Veterinary Medicine<BR>Ghent
University<BR>Salisburylaan 133<BR>B9820 Merelbeke - Belgium<BR>Tel 0032(0)9
264 7745<BR>Fax 0032(0)9 264 7789</FONT></DIV></BLOCKQUOTE></BODY></HTML>