[Histonet] need help for epon

[Myri37 <@t> aol.com]

hello
i have embedded thin tissue in epon, after postfixation with osmium
i stuck sections of 5 um on slides with haupt's solution, deplastized in 
ethoxide of sodium in heat for 30 minutes
and after that stained Goldner's trichrome
tissue was  not coloured, and i dont understand why, is it because of osmium 
? 
pleaze do you have a protocol of Goldner's trichrome especially for epon not 
for parrafin sections, or another stain to show collagen and mineralized tissu 
?
Myriam
Natural implant
France
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