[Histonet] RE: CD21

[Drew Sally A.]

We do not use HIER, we use Ventana's protease 1 for 8" with 
BioCare Medical's CD21...have you tried reducing the protease time 
or diluting the  antibody more?  Maybe a different clone would
please them more...

Sally Ann Drew-MT(ASCP)
Univ. of Wisconsin Hospital & Clinics
IHC/ISH Laboratory 
600 Highland Ave. VAH-DM223
Madison, WI 53792-2472
608-265-6596
sa.drew <@t> hosp.wisc.edu


-----Original Message-----
From: Mcleod [mailto:hmcleod <@t> chempath.uct.ac.za]
Sent: Tuesday, November 11, 2003 8:03 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD21


Dear All
We use the Dakocytomation (M0784) CD21 with 10mins Protease AR on the
Ventana Nexes - IVIEW DAB.  We get epthelial staining as well as the
expected pattern of staining.  The spec sheet states that this does
occur.  The problem is our path's are not happy.  Is anybody using
HIER.  If yes what dilutions are used? 

Thanks for all answers.
Heather

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