<HTML><FONT FACE=arial,helvetica><FONT SIZE=2 FAMILY="SANSSERIF" FACE="Arial" LANG="1">hello<BR>
i have embedded thin tissue in epon, after postfixation with osmium<BR>
i stuck sections of 5 um on slides with haupt's solution, deplastized in ethoxide of sodium in heat for 30 minutes<BR>
and after that stained Goldner's trichrome<BR>
tissue was not coloured, and i dont understand why, is it because of osmium ? <BR>
pleaze do you have a protocol of Goldner's trichrome especially for epon not for parrafin sections, or another stain to show collagen and mineralized tissu ?<BR>
Myriam<BR>
Natural implant<BR>
France<BR>
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