[Histonet] sectioning tissue mircroarrays

[louise renton]

Dear Jason,

Could it be that when the cores were insetrd into the final block they were 
not warmed sufficiently? I have seen this "shelling" out of normal tissue 
bocks when there has been a temperature mismatch, ie when the block of 
tissue was cold and a hardened layer of wax had formed, and then embedded in 
normal hot wax. The only remedy under these circumstances was to melt the 
whole thing down and start again.

best regards



Louise Renton
Bone Research Unit
MRC
Johannesburg
South Africa
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"





----Original Message Follows----
From: "jason madore" <kosmicdog <@t> hotmail.com>
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] sectioning tissue mircroarrays
Date: Fri, 07 Nov 2003 06:54:18 -0800

Salut everyone,

I am hoping that someone can give me pointers for sectioning of tissue 
microarrays. I am using leica RM2125 and the first array i need to section 
is a 0.06mm 400core ovarian serous tumour array. I have a problems with the 
cores rolling up as the section is cut. About half of the roled cores 
flatten out in the water bath but still losing half the array in this way is 
not good. Is the knife or some step prior to sectioning. The block was 
incubated at 37ds for half an hour. Any pointers on dealing with these 
problems or any other problems I might encounter would be greatly 
appreciated.
Thanks in advance. I am of course checking the histonet archives as well.

Ciao, J.

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