[Histonet] CD 4/CD 8 staining of frozen stomach sections of mice

[antje.marcantonio <@t> pharma.novartis.com]

Hello Manuelle

may I ask you to check your secondary antibody? How does the control look 
like without applying the primary ? 
We use both for CD4 and for CD8a on mouse frozens antibodies( host rat) 
from Pharmigen.  As secondary I use a rabbit anti-rat biotinylated one, mouse adsorbed from Vector.
For the blocking of endogenous peroxidase don't use methanol (!) , it 
weakens the signal in most cases. For the unspecific binding block we use 
10% normal rabbit serum (-> secondary host species).
I hope this helps.
Good luck,

Antje Marcantonio
Novartis Pharma AG
BU Transplantation Research
Basel, Switzerland
Tel. +41 61 324 6730
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