[Histonet] What am I going to do?

[louise renton]

Dear Jenny,

this might be absolutely no help to you at all, but I have NEVER had any 
sucess in keeping my MMA sections on the slide. I have tried Haupt's 
solution, chrome gelatin (only worked at high concentrations that left 
++++background on slide), Superfrost and APES coated slides. So we come back 
to the old tried and test and , if truth be told, a little iffy, method of 
staining the sections free - floating ( we use ordinary processing casettes 
for this) and then mounting them from abs alcohol  with Entellan.  Of 
course, with this method, IMP is probably impossible, but tinctorial 
staining works fine if with a little tweaking.
If you want a more detailed method, let me know and I will send you one

best rgards
Louise Renton
Bone Research Unit
MRC
Johannesburg
South Africa
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"





----Original Message Follows----
From: Jenny Molde <ctsmolde <@t> capeheart.uct.ac.za>
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] What am I going to do?
Date: Fri, 7 Nov 2003 14:12:16 +0200

Hi everyone out there
Yes it`s me again. I have not been able to keep the stented sections on my 
slides. I have tried all the suggestions made by you wonderful people out 
there. It`s time to take early retirement as I have no other options left 
now. Thanks all for your input but none of it worked. Do any of you know if 
methylmethacrylate goes off  for any reason? This is my last resort.  Many 
thanks in advance.

Jenny Molde
Cardiovascular Research Unit
University of Cape Town
South Africa

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