[Histonet] Not all tissue staining...

Tue Nov 4 13:48:27 CST 2003

I just had that same problem resolved yesterday.  I don't know if you do it too, but we cut the benchmark labels to prevent them from lifting from the unpainted part of the slide.  We only cut off the little tab that has the lot no. on it.  Yesterday I was in a hurry and my cuts were not as smooth as they should have been.  What does that have to do with my problem you ask?  If you remember from benchmark training, the solution on top of the slide creates a "dome" over the tissue section, now if the edge of the dome touches an uneven label or worst a ragged edge label, the surface tension of the dome is broken and the solution will drain down the side of your slide.  I am willing to bet that you had good staining closest to the label.  That is because some of the solution stays behind, but the rest of the silde dries up.  I had one slide drain so fast that even the hematoxylin didn't stainned it.  
Like somebody mentioned before, It's always good to give Ventana a call, they are the ones who helped me through this.  Good luck and sorry for rambling on.

Juan C. Gutierrez, HT(ASCP)

	-----Original Message----- 
	From: Willis, Donna [mailto:DonnaWillis <@t> texashealth.org] 
	Sent: Tue 11/4/2003 1:06 PM 
	To: 'ANN MARUSKA'; usdahisto <@t> hotmail.com; laurie.colbert <@t> huntingtonhospital.com; histonet <@t> lists.utsouthwestern.edu; Bauer.Karen <@t> mayo.edu 
	Cc: 
	Subject: RE: [Histonet] Not all tissue staining...

	We had the same problem and found out the hard way that it was the slides we
	were using.  We were using Gold Plus Slides.  They worked great on the Nexes
	but when we converted to the Benchmark and XT with the new solutions we had
	staining issues.  We sent slides inhouse to Ventana and they researched the
	issue.  It wasn't the instruments it was the solutions with the slides.  If
	the slides went through Xylene and Alcohols on a automatic stainer there
	wasn't a problem but if we put them through the EX Prep and CC1 then the
	problem occured.  We went to using Plus Slides and everything is wonderful.

	Donna Willis
	Histology Lab Manager
	Harris Methodist Fort Worth, Tx

	-----Original Message-----
	From: ANN MARUSKA [mailto:amarusk1 <@t> FAIRVIEW.ORG]
	Sent: Tuesday, November 04, 2003 12:27 PM
	To: usdahisto <@t> hotmail.com; laurie.colbert <@t> huntingtonhospital.com;
	histonet <@t> lists.utsouthwestern.edu; Bauer.Karen <@t> mayo.edu
	Subject: RE: [Histonet] Not all tissue staining...

	Karen,

	I am with you - I would suspect the vortex mixers first.  But perhaps
	deparaffinization is a problem.  It is possible that the volume of EZ prep
	that goes on the slide - and deparaffinizes - might be the problem....or
	could it have been made up improperly......or the line have a bubble in it
	that needs to be primed.

	I agree that contacting technical service at Ventana would be the first step
	in having them walk you thru these procedures and troubleshoot.

	I've had my Benchmark for 3 years come this January - and have no
	complaints.

	Ann

	Ann Maruska
	Fairview-University Medical Center
	Mpls. MN  55454
	amarusk1 <@t> fairview.org <mailto:amarusk1 <@t> fairview.org>
	612-273-9119

	>>> "T. Truscott" <usdahisto <@t> hotmail.com> 11/04/03 11:56AM >>>
	Karen, Ventana can be great help, but you can also check that all your
	reagent containers are clean and that LCS didn't get poured into the wrong
	one. Good Luck Tom Truscott USDA-ARS Pullman, WA

	>From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
	>To: "Bauer, Karen" <Bauer.Karen <@t> mayo.edu>,"Histonet (E-mail)"
	><histonet <@t> lists.utsouthwestern.edu>
	>Subject: RE: [Histonet] Not all tissue staining...
	>Date: Wed, 29 Oct 2003 10:47:26 -0800
	>
	>Karen,
	>
	>We have four BenchMarks and and a NexES special stainer and whenever we
	>have a problem we call Ventana's customer service.  If they can't
	>troubleshoot over the phone, they are very willing to send a service tech
	>out.
	>
	>-----Original Message-----
	>From: Bauer, Karen [ mailto:Bauer.Karen <@t> mayo.edu]
	<mailto:Bauer.Karen <@t> mayo.edu]>
	>Sent: Wednesday, October 29, 2003 10:25 AM
	>To: Histonet (E-mail)
	>Subject: [Histonet] Not all tissue staining...
	>
	>
	>Hello to all,
	>
	>There are times when a stained IP slide will have only half of the tissue
	>staining positively and not other parts of the tissue, but has the same
	>cellularity of the part that is staining. (Did that make sense?)  Same
	>tissue cells, but some stain and some do not.  When the Pathologist asks
	>why
	>this happens, I can only come up with possible vortex mixing problems or
	>that the paraffin did not come out completely.  We've recently switched to
	>the BenchMark and this has only happened a few times.  I've checked the
	>vortex mixers, and all of the heating pads are working properly.
	>
	>Does anyone have any suggestions?
	>
	>Thanks in advance.
	>
	>Karen L. Bauer HT(ASCP)
	>Histology Department
	>Luther Hospital
	>715-838-3205
	>
	>
	>
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