[Histonet] RE: dystrophin

[C.M. van der Loos]

Dear Heather,
If you don't see anything after testing these dystrophin antibodies on 
FFPE tissues you may try the following (as last resort perhaps):
- Good old pepsin digestion (instead of HIER) using 0.25% pepsin 
(Sigma) in 10 mM HCl. 15 min at 37C. 
- A stronger detection system. Try for example the new CSA II kit from 
DakoCyto. A non-biotin based peroxidase staining kit that is very 
powerful. Test your antibodies in steps like: 1:50 - 200 - 1000 - 5000.
Good luck,

Chris van der Loos
Academical Medical Center
Dept. of Cardiovascular Pathology
Amsterdam - The Netherlands

----- Original Message ----- 
>From  Mcleod <hmcleod <@t> chempath.uct.ac.za> 
Date  Tue, 04 Nov 2003 07:15:44 +0200 
To  "histonet <@t> lists.utsouthwestern.edu" 
<histonet <@t> lists.utsouthwestern.edu> 
Subject  [Histonet] dystrophin 

------------------------------------------------------------------------

Dear All

Would anybody be able to help with the above antibody? A researcher who
has never done any IHC before bought the following Novocastra Antobodies
(1) NCL-DYS1 (2) NCL-DYS2 and (3) NCL-DYS3.  These however are for
frozen sections and she needs to work with FFPE material.  A first
attempt on the ffpe sections gave no staining. Has anybody by any chance
ever had the need to use them?  ANY help will be greatly appreciated.
Thankyou
Heather McLeod