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<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=124062818-17122003>I use
the B&D too, and I just fill the steamer fuller than you are supposed
to..... almost to the top, and it always has enough for preheating and the
longest time I use (40 min). The other thing I do sometimes (more for
speed than for the reason you mention) is to heat the buffer in the microwave
for a bit first. Then the preheating time is less than 20
minutes.</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=124062818-17122003>
<P><FONT face=Papyrus size=2>Bonnie Whitaker<BR>University of Texas Medical
School at Houston<BR>6431 Fannin Street<BR>MSB 2.231<BR>Houston, Texas
77030<BR>Phone 713.500.6792<BR>Fax 713.500.0733 </FONT></P></SPAN></FONT></DIV>
<BLOCKQUOTE>
<DIV class=OutlookMessageHeader dir=ltr align=left><FONT face=Tahoma
size=2>-----Original Message-----<BR><B>From:</B>
histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-admin@lists.utsouthwestern.edu]<B>On Behalf Of </B>Tan,
MinHan<BR><B>Sent:</B> Wednesday, December 17, 2003 12:13 PM<BR><B>To:</B> Kim
Merriam; Bartlett, Jeanine;
histonet@lists.utsouthwestern.edu<BR><B>Subject:</B> RE: [Histonet]
steamers<BR><BR></FONT></DIV>
<DIV><SPAN class=460021118-17122003><FONT face=Arial color=#0000ff size=2>Yes,
we use that too. Only problem is that for long ARs (30-35 min), it tends
to run out of water if not topped up - it takes about 15-20 min to bring the
temp of citrate buffer in a Coplin jar up to 95 deg C.</FONT></SPAN></DIV>
<DIV><SPAN class=460021118-17122003><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=460021118-17122003><FONT face=Arial color=#0000ff
size=2>Min-han Tan</FONT></SPAN></DIV>
<DIV><SPAN class=460021118-17122003><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=460021118-17122003><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<BLOCKQUOTE style="MARGIN-RIGHT: 0px">
<DIV></DIV>
<DIV class=OutlookMessageHeader lang=en-us dir=ltr align=left><FONT
face=Tahoma size=2>-----Original Message-----<BR><B>From:</B> Kim Merriam
[mailto:kmerriam2003@yahoo.com] <BR><B>Sent:</B> Wednesday, December 17,
2003 12:04 PM<BR><B>To:</B> Bartlett, Jeanine;
histonet@lists.utsouthwestern.edu<BR><B>Subject:</B> Re: [Histonet]
steamers<BR><BR></FONT></DIV>
<DIV>I just use a Black & Decker Flavor Scenter Handy Steamer.
About 30 bucks at Walmart!</DIV>
<DIV> </DIV>
<DIV>Kim Merriam</DIV>
<DIV>Novartis</DIV>
<DIV>Cambridge, MA<BR><BR><B><I>"Bartlett, Jeanine"
<jqb7@cdc.gov></I></B> wrote:</DIV>
<BLOCKQUOTE class=replbq
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<DIV><SPAN class=330250916-17122003><FONT face=Arial
size=2>All:</FONT></SPAN></DIV>
<DIV><SPAN class=330250916-17122003><FONT face=Arial
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=330250916-17122003><FONT face=Arial size=2>Can anyone
recommend a good, laboratory-grade steamer for antigen retrieval?
</FONT></SPAN></DIV>
<DIV><SPAN class=330250916-17122003><FONT face=Arial
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=330250916-17122003><FONT face=Arial
size=2>Thanks!</FONT></SPAN></DIV>
<DIV><SPAN class=330250916-17122003>
<DIV align=left><FONT face=Arial size=2>Jeanine Bartlett,
HT(ASCP)</FONT></DIV>
<DIV align=left><FONT face=Arial size=2>Centers for Disease
Control</FONT></DIV>
<DIV align=left><FONT face=Arial size=2>Infectious Disease Pathology
Activity</FONT></DIV>
<DIV align=left><FONT face=Arial size=2>1600 Clifton Road,
MS/G-32</FONT></DIV>
<DIV align=left><FONT face=Arial size=2>Atlanta, GA
30333</FONT></DIV></SPAN></DIV></BLOCKQUOTE>
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