[Histonet] gomori methenamin silver
jqb7 <@t> cdc.gov
Wed Dec 17 08:32:06 CST 2003
We use the Grocott's Method.
Stock Solution A: 0.21% Silver nitrate (2.1 grams silver nitrate with
1000 ml distilled water): make in chemically clean glassware and
Stock Solution B: Methenamine-borax (27.0 grams methenamine, 3.8 grams
borax, 1000 ml distilled water): make in chemically clean glassware and
Both stock solutions are stable for up to at least 4 months. We use
equal portions of each for our working solution. We do not have a
problem with sediment and the staining is just as good at 4 months as it
is earlier. We haven't tried it beyond that time period because we
always have it used up by then. I think the methenamine gives the
silver the alkaline properties needed for a proper reaction.
Jeanine Bartlett, HT(ASCP)
Centers for Disease Control
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333
From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun
Sent: Wednesday, December 17, 2003 9:17 AM
Subject: [Histonet] gomori methenamin silver
I need help of those, who are familiar to Gomori Methenamin
I have troubles to convince my colleages about the advantages of
using a stock meth-silver-solution. (instead of fresh making each time)
stock: 1:40 10%Silvernitrate : 3% Hexamethenamin (stable for
working: 1:10 Borax : stock
My colleages think, that the stock solution soon becomes
instable. And that there is a kind of sediment at the bottom of the
bottle (more concentration of silver).
Can anyone tell me about bad experience with older stock
solutions? What happens, when the stock solution is older than two (or
three,..) months? Is the result of the stain weaker or darker?
What is the the function of Hexamethenamin? I know it causes the
high pH, but does it make any compound with the silvernitrate?
Any help is welcome
Best wishes from Austria
Akh Linz, Austria
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