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<DIV><SPAN class=632392314-17122003><FONT face=Arial color=#0000ff size=2>We use
the Grocott's Method.</FONT></SPAN></DIV>
<DIV><SPAN class=632392314-17122003><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=632392314-17122003><FONT face=Arial color=#0000ff size=2>Stock
Solution A: 0.21% Silver nitrate (2.1 grams silver nitrate with 1000 ml
distilled water): make in chemically clean glassware and
refrigerate</FONT></SPAN></DIV>
<DIV><SPAN class=632392314-17122003><FONT face=Arial color=#0000ff size=2>Stock
Solution B: Methenamine-borax (27.0 grams methenamine, 3.8 grams borax, 1000 ml
distilled water): make in chemically clean glassware and
refrigerate</FONT></SPAN></DIV>
<DIV><SPAN class=632392314-17122003><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=632392314-17122003><FONT face=Arial color=#0000ff size=2>Both
stock solutions are stable for up to at least 4 months. We use equal
portions of each for our working solution. We do not have a problem with
sediment and the staining is just as good at 4 months as it is earlier. We
haven't tried it beyond that time period because we always have it used up by
then. I think the methenamine gives the silver the alkaline properties
needed for a proper reaction.</FONT></SPAN></DIV>
<DIV><SPAN class=632392314-17122003><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=632392314-17122003>
<DIV align=left><FONT face=Arial size=2>Jeanine Bartlett, HT(ASCP)</FONT></DIV>
<DIV align=left><FONT face=Arial size=2>Centers for Disease Control</FONT></DIV>
<DIV align=left><FONT face=Arial size=2>Infectious Disease Pathology
Activity</FONT></DIV>
<DIV align=left><FONT face=Arial size=2>1600 Clifton Road, MS/G-32</FONT></DIV>
<DIV align=left><FONT face=Arial size=2>Atlanta, GA
30333</FONT></DIV></SPAN></DIV>
<DIV><SPAN class=632392314-17122003><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<BLOCKQUOTE dir=ltr style="MARGIN-RIGHT: 0px">
<DIV></DIV>
<DIV class=OutlookMessageHeader lang=en-us dir=ltr align=left><FONT
face=Tahoma size=2>-----Original Message-----<BR><B>From:</B>
histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-admin@lists.utsouthwestern.edu] <B>On Behalf Of </B>Gudrun
Lang<BR><B>Sent:</B> Wednesday, December 17, 2003 9:17 AM<BR><B>To:</B>
Histonetliste<BR><B>Subject:</B> [Histonet] gomori methenamin
silver<BR><BR></FONT></DIV>
<DIV><FONT face=Arial size=2>Dear histonetters,</FONT></DIV>
<DIV><FONT face=Arial size=2>I need help of those, who are familiar to Gomori
Methenamin Silver staining.</FONT></DIV>
<DIV><FONT face=Arial size=2>I have troubles to convince my colleages about
the advantages of using a stock meth-silver-solution. (instead of fresh making
each time)</FONT></DIV>
<DIV><FONT face=Arial size=2>stock: 1:40 10%Silvernitrate :
3% Hexamethenamin (stable for few months); </FONT></DIV>
<DIV><FONT face=Arial size=2>working: 1:10 Borax : stock</FONT></DIV>
<DIV><FONT face=Arial size=2>My colleages think, that the stock solution
soon becomes instable. And that there is a kind of sediment at the bottom
of the bottle (more concentration of silver).</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Can anyone tell me about bad experience with
older stock solutions? What happens, when the stock solution is older than two
(or three,..) months? Is the result of the stain weaker or
darker?</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Question 2:</FONT></DIV>
<DIV><FONT face=Arial size=2>What is the the function of Hexamethenamin? I
know it causes the high pH, but does it make any compound with
the silvernitrate?</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Any help is welcome</FONT></DIV>
<DIV><FONT face=Arial size=2>Best wishes from Austria</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Gudrun Lang</FONT></DIV>
<DIV><FONT face=Arial size=2>Akh Linz,
Austria</FONT></DIV></BLOCKQUOTE></BODY></HTML>