[Histonet] Make of Toluidine blue solution

Lina Vieira livieira <@t> ualg.pt
Tue Dec 9 08:47:00 CST 2003

Helo Everyone,

In our lab usually, we make the toluidine solution (for coloration of resin included tissue): 
1g - Borax
1g  - Toluidine Blue
100 - ml water,

1 minute - staining time

And because, now, I have another protocol for make the solution, I want ask you about the possibility to use this solution, and if is it necessary change the staining time?

 New protocol:
0.2 g - Toluidine Blue
0.5 - Acetic acid
Complete, with desionized water, to 100 ml

Tanks in advance

Lina Vieira

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