[Histonet] Make of Toluidine blue solution

[Geoff McAuliffe]

Hi Lina:

    The borax makes the stain alkaline so it will penetrate the epoxy. 
Acetic acid will make the stain acidic, I don't know how well it will 
penetrate the epoxy (probably not very well which is why people have 
been putting borax in their toluidine blue since 1961 or so). If you 
have a protocol that works, why change?

Geoff

Lina Vieira wrote:

> Helo Everyone,
>  
> In our lab usually, we make the toluidine solution (for coloration of 
> resin included tissue):
> 1g - Borax
> 1g  - Toluidine Blue
> 100 - ml water,
>  
> 1 minute - staining time
>  
> And because, now, I have another protocol for make the solution, I 
> want ask you about the possibility to use this solution, and if is it 
> necessary change the staining time?
>  
>  New protocol:
> 0.2 g - Toluidine Blue
> 0.5 - Acetic acid
> Complete, with desionized water, to 100 ml
>  
>  
> Tanks in advance
>  
> Lina Vieira
>  


-- 
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**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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