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<DIV><FONT face=Arial size=2>Helo Everyone,</FONT></DIV>
<DIV> </DIV>
<DIV>In our lab usually, we make the toluidine solution (for coloration of resin
included tissue): </DIV>
<DIV>1g - Borax</DIV>
<DIV>1g - Toluidine Blue</DIV>
<DIV>100 - ml water,</DIV>
<DIV> </DIV>
<DIV>1 minute - staining time</DIV>
<DIV> </DIV>
<DIV>And because, now, I have another protocol for make the solution, I want ask
you about the possibility to use this solution, and if is it necessary
change the staining time?</DIV>
<DIV> </DIV>
<DIV> New protocol:</DIV>
<DIV>0.2 g - Toluidine Blue</DIV>
<DIV>0.5 - Acetic acid</DIV>
<DIV>Complete, with desionized water, to 100 ml</DIV>
<DIV> </DIV>
<DIV> </DIV>
<DIV><FONT face=Arial size=2>Tanks in advance</FONT></DIV>
<DIV> </DIV>
<DIV>Lina Vieira</DIV>
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