[Histonet] fixed frozen IHC

Hernan Aldana Marcos haldana <@t> unimoron.edu.ar
Fri Dec 5 11:53:02 CST 2003

You have a lot of advaices in the wonderfull manual of Technical
Immunohistochemistry of Rodney Miller, go to http://www.propathlab.com and
download it free.Or go to
"http://hjaldanamarcos.bravepages.com/documentos.htm"  and download the
documents named INMUNO1.... to INMUNO5. Be patient becouse this web pages
are free and if a lot of people download the archives the pages interrupt
temporarly (continue in other day)

Dr. Hernán J. Aldana Marcos
Facultad de Medicina. Universidad de Morón
Machado 914. B1708JPD. Buenos Aires. Argentina
e-mail alternativo hernanjavier <@t> yahoo.com
web: http://hjaldanamarcos.bravepages.com

----- Original Message -----
From: "Gehan, Loralee" <Loralee_Gehan <@t> URMC.Rochester.edu>
To: <histonet <@t> pathology.swmed.edu>
Sent: Friday, December 05, 2003 1:46 PM
Subject: [Histonet] fixed frozen IHC

> I think that this was already discussed a few weeks ago so sorry for the
> repeat.
> I need to do some immunohistochemistry on fixed (some in gluteraldehyde,
> in formalin)  frozen sections.  Does anyone have any tricks to make some
> the more tricky antibodies work? Suggestions for Antigen retrieval?
> Thanks in advance.
> Loralee Gehan
> University of Rochester
> Orthopaedics Research Department
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