[Histonet] fixed frozen IHC

Gehan, Loralee Loralee_Gehan <@t> URMC.Rochester.edu
Fri Dec 5 10:46:32 CST 2003

I think that this was already discussed a few weeks ago so sorry for the

I need to do some immunohistochemistry on fixed (some in gluteraldehyde, so
in formalin)  frozen sections.  Does anyone have any tricks to make some of
the more tricky antibodies work? Suggestions for Antigen retrieval?  
Thanks in advance. 

Loralee Gehan
University of Rochester
Orthopaedics Research Department

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