[Histonet] Stain for H.Pylori

Wed Dec 3 12:20:16 CST 2003

MODIFICATION OF GIEMSA STAIN FOR HELICOBACTER

FIXATION: Neutral Formolin   10%

SECCIONS: 4-5 µm

SOLUTIONS:

ACETIC WATER

Acetic acid............... 0,01 ml

Destilated water............... 1000,00 ml

Working solution GIEMSA

Giemsa.............. 10,0 ml

Acetic water.............. 50,0 ml

PROCEDDURE

1.     Take slides to water destiled

2.     Giemsa working solution 1 h

3.     Deshidratation in 3 changes of alcohol 100

4.     xilol, syntetic mounting medium

RESULTADOS

H. pylorii.............. intense blue

Nuclei.............. blue

Cytoplasm.............. Rose

REFERENCE

Luna L.: "Wet work shop for special Alabama" Noviembre 2 de 1984

Dr. Hernán J. Aldana Marcos
Facultad de Medicina. Universidad de Morón
Machado 914. B1708JPD. Buenos Aires. Argentina 
e-mail alternativo hernanjavier <@t> yahoo.com
web: http://hjaldanamarcos.bravepages.com
http://histologia.bigthicketdirectory.net/main.html

  ----- Original Message ----- 
  From: Jill Cox 
  To: histonet <@t> lists.utsouthwestern.edu 
  Sent: Wednesday, December 03, 2003 1:58 PM
  Subject: [Histonet] Stain for H.Pylori

  Hello everyone,
   Has anyone done a Toluidine blue/Alcian yellow stain for H. Pylori? If so how do the Doctors like it?   Or, what other stains are you doing for that. I am trying to get away from the Genta!! Thanks in advance

  Jill Cox HT (ASCP) 
  Histology Supervisor
  Seattle Histology Lab

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