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<P class=MsoNormal style="MARGIN: 0pt; TEXT-ALIGN: center" align=center><SPAN
lang=ES-TRAD style="FONT-SIZE: 16pt; mso-bidi-font-size: 10.0pt">MODIFICATION OF
GIEMSA STAIN FOR HELICOBACTER<?xml:namespace prefix = o ns =
"urn:schemas-microsoft-com:office:office" /><o:p></o:p></SPAN></P>
<P class=MsoNormal style="MARGIN: 0pt"><SPAN lang=ES-TRAD><FONT size=2>FIXATION:
Neutral Formolin 10%</FONT></SPAN></P>
<P class=MsoNormal style="MARGIN: 0pt"><SPAN lang=ES-TRAD><FONT size=2>SECCIONS:
4-5 µm</FONT></SPAN></P>
<P class=MsoNormal style="MARGIN: 0pt"><SPAN lang=ES-TRAD><FONT
size=2> <o:p></o:p></FONT></SPAN></P>
<P class=MsoNormal style="MARGIN: 0pt; TEXT-ALIGN: center" align=center><B
style="mso-bidi-font-weight: normal"><SPAN lang=ES-TRAD
style="FONT-SIZE: 14pt; mso-bidi-font-size: 10.0pt">SOLUTIONS:<o:p></o:p></SPAN></B></P>
<P class=MsoNormal style="MARGIN: 0pt; tab-stops: decimal dotted 354.4pt"><B
style="mso-bidi-font-weight: normal"><SPAN lang=ES-TRAD><FONT size=2>ACETIC
WATER</FONT></SPAN></B></P>
<P class=MsoNormal
style="MARGIN: 0pt 0pt 0pt 70.8pt; tab-stops: decimal dotted 354.4pt"><SPAN
lang=ES-TRAD><FONT size=2>Acetic acid<SPAN
style="mso-tab-count: 1 dotted">............... </SPAN>0,01 ml</FONT></SPAN></P>
<P class=MsoNormal
style="MARGIN: 0pt 0pt 0pt 70.8pt; tab-stops: decimal dotted 354.4pt"><SPAN
lang=ES-TRAD><FONT size=2><SPAN style="mso-tab-count: 1 dotted">Destilated
water............... </SPAN>1000,00 ml</FONT></SPAN></P>
<P class=MsoNormal
style="MARGIN: 0pt 0pt 0pt 70.8pt; tab-stops: decimal dotted 354.4pt"><SPAN
lang=ES-TRAD><FONT size=2> <o:p></o:p></FONT></SPAN></P>
<P class=MsoNormal style="MARGIN: 0pt; tab-stops: decimal dotted 354.4pt"><B
style="mso-bidi-font-weight: normal"><SPAN lang=ES-TRAD><FONT size=2>Working
solution GIEMSA<o:p></o:p></FONT></SPAN></B></P>
<P class=MsoNormal
style="MARGIN: 0pt 0pt 0pt 70.9pt; tab-stops: decimal dotted 354.4pt"><SPAN
lang=ES-TRAD><FONT size=2>Giemsa<SPAN
style="mso-tab-count: 1 dotted">.............. </SPAN>10,0 ml</FONT></SPAN></P>
<P class=MsoNormal
style="MARGIN: 0pt 0pt 0pt 70.9pt; tab-stops: decimal dotted 354.4pt"><SPAN
lang=ES-TRAD><FONT size=2>Acetic water<SPAN
style="mso-tab-count: 1 dotted">.............. </SPAN>50,0 ml</FONT></SPAN></P>
<P class=MsoNormal
style="MARGIN: 0pt 0pt 0pt 70.9pt; tab-stops: decimal dotted 354.4pt"><SPAN
lang=ES-TRAD><FONT size=2> <o:p></o:p></FONT></SPAN></P>
<P class=MsoNormal style="MARGIN: 0pt"><B
style="mso-bidi-font-weight: normal"><SPAN lang=ES-TRAD><FONT
size=2>PROCEDDURE</FONT></SPAN></B></P>
<P class=MsoNormal style="MARGIN: 0pt 0pt 0pt 18pt; TEXT-INDENT: -18pt"><SPAN
lang=ES-TRAD><FONT size=2>1.<SPAN
style="mso-tab-count: 1"> Take slides to water
destiled</SPAN></FONT></SPAN></P>
<P class=MsoNormal style="MARGIN: 0pt 0pt 0pt 18pt; TEXT-INDENT: -18pt"><SPAN
lang=ES-TRAD><FONT size=2>2.<SPAN
style="mso-tab-count: 1"> Giemsa working solution 1
h</SPAN></FONT></SPAN></P>
<P class=MsoNormal style="MARGIN: 0pt 0pt 0pt 18pt; TEXT-INDENT: -18pt"><SPAN
lang=ES-TRAD><FONT size=2>3.<SPAN
style="mso-tab-count: 1"> </SPAN>Deshidratation in 3
changes of alcohol 100</FONT></SPAN></P>
<P class=MsoNormal style="MARGIN: 0pt 0pt 0pt 18pt; TEXT-INDENT: -18pt"><SPAN
lang=ES-TRAD><FONT size=2>4.<SPAN
style="mso-tab-count: 1"> </SPAN>xilol, syntetic
mounting medium</FONT></SPAN></P>
<P class=MsoNormal style="MARGIN: 0pt 0pt 0pt 18pt; TEXT-INDENT: -18pt"><SPAN
lang=ES-TRAD><FONT size=2> <o:p></o:p></FONT></SPAN></P>
<P class=MsoNormal style="MARGIN: 0pt"><B
style="mso-bidi-font-weight: normal"><SPAN lang=ES-TRAD><FONT
size=2>RESULTADOS<o:p></o:p></FONT></SPAN></B></P>
<P class=MsoNormal
style="MARGIN: 0pt 0pt 0pt 106.35pt; tab-stops: right dotted 318.95pt"><SPAN
lang=ES-TRAD><FONT size=2>H. pylorii<SPAN
style="mso-tab-count: 1 dotted">.............. intense
blue</SPAN></FONT></SPAN></P>
<P class=MsoNormal
style="MARGIN: 0pt 0pt 0pt 106.35pt; tab-stops: right dotted 318.95pt"><SPAN
lang=ES-TRAD><FONT size=2>Nuclei<SPAN
style="mso-tab-count: 1 dotted">.............. blue</SPAN></FONT></SPAN></P>
<P class=MsoNormal
style="MARGIN: 0pt 0pt 0pt 106.35pt; tab-stops: right dotted 318.95pt"><SPAN
lang=ES-TRAD><FONT size=2>Cytoplasm<SPAN
style="mso-tab-count: 1 dotted">.............. </SPAN>Rose</FONT></SPAN></P>
<P class=MsoNormal
style="MARGIN: 0pt 0pt 0pt 106.35pt; tab-stops: right dotted 318.95pt"><SPAN
lang=ES-TRAD><FONT size=2> <o:p></o:p></FONT></SPAN></P>
<P class=MsoNormal style="MARGIN: 0pt"><B
style="mso-bidi-font-weight: normal"><SPAN lang=ES-TRAD><FONT
size=2>REFERENCE</FONT></SPAN></B></P><SPAN lang=ES-TRAD
style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: ES-TRAD; mso-fareast-language: ES; mso-bidi-language: AR-SA">Luna
L.: "Wet work shop for special Alabama" Noviembre 2 de 1984</SPAN></DIV>
<DIV> </DIV>
<DIV> </DIV>
<DIV>Dr. Hernán J. Aldana Marcos<BR>Facultad de Medicina. Universidad de
Morón<BR>Machado 914. B1708JPD. Buenos Aires. Argentina <BR>e-mail alternativo
<A href="mailto:hernanjavier@yahoo.com">hernanjavier@yahoo.com</A><BR>web: <A
href="http://hjaldanamarcos.bravepages.com">http://hjaldanamarcos.bravepages.com</A><BR><A
href="http://histologia.bigthicketdirectory.net/main.html">http://histologia.bigthicketdirectory.net/main.html</A><BR></DIV>
<BLOCKQUOTE
style="PADDING-RIGHT: 0px; PADDING-LEFT: 5px; MARGIN-LEFT: 5px; BORDER-LEFT: #000000 2px solid; MARGIN-RIGHT: 0px">
<DIV style="FONT: 10pt arial">----- Original Message ----- </DIV>
<DIV
style="BACKGROUND: #e4e4e4; FONT: 10pt arial; font-color: black"><B>From:</B>
<A title=jcox90@yahoo.com href="mailto:jcox90@yahoo.com">Jill Cox</A> </DIV>
<DIV style="FONT: 10pt arial"><B>To:</B> <A
title=histonet@lists.utsouthwestern.edu
href="mailto:histonet@lists.utsouthwestern.edu">histonet@lists.utsouthwestern.edu</A>
</DIV>
<DIV style="FONT: 10pt arial"><B>Sent:</B> Wednesday, December 03, 2003 1:58
PM</DIV>
<DIV style="FONT: 10pt arial"><B>Subject:</B> [Histonet] Stain for
H.Pylori</DIV>
<DIV><BR></DIV>
<DIV>Hello everyone,</DIV>
<DIV> Has anyone done a Toluidine blue/Alcian yellow stain for H. Pylori?
If so how do the Doctors like it? Or, what other stains are you
doing for that. I am trying to get away from the Genta!! Thanks in
advance</DIV><BR><BR>
<DIV>
<DIV>Jill Cox HT (ASCP) </DIV>
<DIV>Histology Supervisor<BR>Seattle Histology Lab</DIV></DIV>
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