[Histonet] Stain for H.Pylori

[Lee & Peggy Wenk]

How does one make up the "stock" Giemsa, which is then diluted to make the working? 
- concentration? (X grams Giemsa powder to Y grams solvent?)
- pH? (with what - acetic acid? buffer? Which one?)
- aqueous or alcoholic?
- if alcoholic, what %?? which alcohol? (ethanol? methanol?)
- etc.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

  ----- Original Message ----- 
  From: Hernan Aldana Marcos 
  To: Jill Cox 
  Cc: histonet <@t> lists.utsouthwestern.edu 
  Sent: Wednesday, December 03, 2003 1:20 PM
  Subject: Re: [Histonet] Stain for H.Pylori


  MODIFICATION OF GIEMSA STAIN FOR HELICOBACTER

  FIXATION: Neutral Formolin   10%

  SECCIONS: 4-5 µm

   

  SOLUTIONS:

  ACETIC WATER

  Acetic acid............... 0,01 ml

  Destilated water............... 1000,00 ml

   

  Working solution GIEMSA

  Giemsa.............. 10,0 ml

  Acetic water.............. 50,0 ml

   

  PROCEDDURE

  1.     Take slides to water destiled

  2.     Giemsa working solution 1 h

  3.     Deshidratation in 3 changes of alcohol 100

  4.     xilol, syntetic mounting medium

   

  RESULTADOS

  H. pylorii.............. intense blue

  Nuclei.............. blue

  Cytoplasm.............. Rose

   

  REFERENCE

  Luna L.: "Wet work shop for special Alabama" Noviembre 2 de 1984


  Dr. Hernán J. Aldana Marcos
  Facultad de Medicina. Universidad de Morón
  Machado 914. B1708JPD. Buenos Aires. Argentina 
  e-mail alternativo hernanjavier <@t> yahoo.com
  web: http://hjaldanamarcos.bravepages.com
  http://histologia.bigthicketdirectory.net/main.html

    ----- Original Message ----- 
    From: Jill Cox 
    To: histonet <@t> lists.utsouthwestern.edu 
    Sent: Wednesday, December 03, 2003 1:58 PM
    Subject: [Histonet] Stain for H.Pylori


    Hello everyone,
     Has anyone done a Toluidine blue/Alcian yellow stain for H. Pylori? If so how do the Doctors like it?   Or, what other stains are you doing for that. I am trying to get away from the Genta!! Thanks in advance



    Jill Cox HT (ASCP) 
    Histology Supervisor
    Seattle Histology Lab


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