[Histonet] Stain for H.Pylori

Lee & Peggy Wenk lpwenk <@t> covad.net
Fri Dec 5 04:01:17 CST 2003

How does one make up the "stock" Giemsa, which is then diluted to make the working? 
- concentration? (X grams Giemsa powder to Y grams solvent?)
- pH? (with what - acetic acid? buffer? Which one?)
- aqueous or alcoholic?
- if alcoholic, what %?? which alcohol? (ethanol? methanol?)
- etc.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

  ----- Original Message ----- 
  From: Hernan Aldana Marcos 
  To: Jill Cox 
  Cc: histonet <@t> lists.utsouthwestern.edu 
  Sent: Wednesday, December 03, 2003 1:20 PM
  Subject: Re: [Histonet] Stain for H.Pylori


  FIXATION: Neutral Formolin   10%

  SECCIONS: 4-5 µm




  Acetic acid............... 0,01 ml

  Destilated water............... 1000,00 ml


  Working solution GIEMSA

  Giemsa.............. 10,0 ml

  Acetic water.............. 50,0 ml



  1.     Take slides to water destiled

  2.     Giemsa working solution 1 h

  3.     Deshidratation in 3 changes of alcohol 100

  4.     xilol, syntetic mounting medium



  H. pylorii.............. intense blue

  Nuclei.............. blue

  Cytoplasm.............. Rose



  Luna L.: "Wet work shop for special Alabama" Noviembre 2 de 1984

  Dr. Hernán J. Aldana Marcos
  Facultad de Medicina. Universidad de Morón
  Machado 914. B1708JPD. Buenos Aires. Argentina 
  e-mail alternativo hernanjavier <@t> yahoo.com
  web: http://hjaldanamarcos.bravepages.com

    ----- Original Message ----- 
    From: Jill Cox 
    To: histonet <@t> lists.utsouthwestern.edu 
    Sent: Wednesday, December 03, 2003 1:58 PM
    Subject: [Histonet] Stain for H.Pylori

    Hello everyone,
     Has anyone done a Toluidine blue/Alcian yellow stain for H. Pylori? If so how do the Doctors like it?   Or, what other stains are you doing for that. I am trying to get away from the Genta!! Thanks in advance

    Jill Cox HT (ASCP) 
    Histology Supervisor
    Seattle Histology Lab

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