[Histonet] sectioning old GMA blocks

Robert Schoonhoven rschoon <@t> email.unc.edu
Mon Dec 1 12:30:12 CST 2003

GMA is hydrophilic and depending on the storage conditions will either 
gain or lose water (in your case lost).  Depending on the brittleness of 
the block anywhere from 12 to 72 hours in a humidity chamber (read 
container with dH2O dampened gauze).  Do not place the blocks directly 
on your water source.

Robert Schoonhoven

Frank Rosenthal wrote:

>Dear colleagues:
>	Does anyone have experience in sectioning glycol methacrylate tissue blocks
>that are several years old?   It seems difficult to get good sections from
>some of them because they are brittle. (I am using a Hacker microtome with a
>steel knife --section thickness 2 - 6 micrometer.  The embedded tissue is
>from dried inflated lung).
>	Any suggestions?
>	Thanks.
>Frank S. Rosenthal, Ph.D.
>Associate Professor
>Purdue University School of Health Sciences
>1338 Civil Engineering Building
>West Lafayette, IN 47907 USA
>tel: 765-494-0812, fax: 765-496-1377,
>e-mail: frank <@t> purdue.edu
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu

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