[Histonet] sectioning old GMA blocks

[Frank Rosenthal]

Dear colleagues:

	Does anyone have experience in sectioning glycol methacrylate tissue blocks
that are several years old?   It seems difficult to get good sections from
some of them because they are brittle. (I am using a Hacker microtome with a
steel knife --section thickness 2 - 6 micrometer.  The embedded tissue is
from dried inflated lung).

	Any suggestions?

	Thanks.

Frank

*****************************************
Frank S. Rosenthal, Ph.D.
Associate Professor
Purdue University School of Health Sciences
1338 Civil Engineering Building
West Lafayette, IN 47907 USA
tel: 765-494-0812, fax: 765-496-1377,
e-mail: frank <@t> purdue.edu
*****************************************