[Histonet] sectioning old GMA blocks

[Pamela Marcum]

That is the best advise you will get to rehydrate the blocks.  If you over
hydrate the cutting will be too soft and you will need to carefully watch
them in a dessicator to get the right consistency.  However, Bob is right
don't place them directly in water.  Pam Marcum

> -----Original Message-----
> From: histonet-admin <@t> lists.utsouthwestern.edu
> [mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of Robert
> Schoonhoven
> Sent: Monday, December 01, 2003 1:30 PM
> To: Frank Rosenthal
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] sectioning old GMA blocks
>
>
> GMA is hydrophilic and depending on the storage conditions will either
> gain or lose water (in your case lost).  Depending on the brittleness of
> the block anywhere from 12 to 72 hours in a humidity chamber (read
> container with dH2O dampened gauze).  Do not place the blocks directly
> on your water source.
>
> Robert Schoonhoven
>
> Frank Rosenthal wrote:
>
> >Dear colleagues:
> >
> >	Does anyone have experience in sectioning glycol
> methacrylate tissue blocks
> >that are several years old?   It seems difficult to get good
> sections from
> >some of them because they are brittle. (I am using a Hacker
> microtome with a
> >steel knife --section thickness 2 - 6 micrometer.  The embedded tissue is
> >from dried inflated lung).
> >
> >	Any suggestions?
> >
> >	Thanks.
> >
> >Frank
> >
> >*****************************************
> >Frank S. Rosenthal, Ph.D.
> >Associate Professor
> >Purdue University School of Health Sciences
> >1338 Civil Engineering Building
> >West Lafayette, IN 47907 USA
> >tel: 765-494-0812, fax: 765-496-1377,
> >e-mail: frank <@t> purdue.edu
> >*****************************************
> >
> >
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>
>
>
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