[Histonet] PASM problem

[Elizabeth Chlipala]

Hello All
 
We have been working on the Jones stain for kidney basement membranes.
We have our internal control specimen which is a human specimen.  The
other specimens we are staining are rat kidneys.  When we stain the
slides, the human kidney comes out great, very good staining of the
basement membranes, very slight nuclear staining with no precipitate.
The rat kidneys don't look as nice, there is good staining of the
basement membrane, but the nuclear staining is more predominant and
there is intense granular staining in the cytoplasm, which sometimes
looks like a precipitate.  Does anyone have any suggestions?  Thanks in
advance.
 
Liz
 
Elizabeth A. Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592 
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
lizchlipala <@t> premierhistology.com
www.premierhistology.com
 
Ship to Address:
Premier Histology Laboratory
University of Colorado
MCBD, Room A3B40
Boulder, Colorado 80309
 
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