[Histonet] Faded H&E tissue section

[Akemi Allison]

Hi Tony:
I actually gave that tip to Lee back in 1979 when he came to OHSU to give a seminar to the Oregon Histology Society.  I found out that when I was doing GMA and the pathologists didn’t like Gills Hematoxylin. They loved my PAS on GMA so I tried using 1% Periodic Acid before using Harris Hematoxylin for H&E’s on GMA and it turned out beautifully. Guess he shared my tip with the rest of our society. He and I became closer se friends and we shared several tips, including his Movat’s tips which he didn’t publish, but I shared them in Frieda’s 4th Ed.

Best,
Akemi Allison-Tacha, BS, HT/HTL (ASCP)

Sent from my iPhone

> On Nov 9, 2023, at 6:11 PM, jayalakshmy p.s via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> Thanks, I'll check it out.
> 
>> On Fri, Nov 10, 2023, 6:58 AM Tony Henwood <afhenwood at outlook.com> wrote:
>> 
>> You could treat the decoverslipped section in 1% periodic acid (same as
>> used in the PAS technique)  for 30 minutes prior to H&E staining. This
>> might improve the H&E staining.
>> 
>> 
>> 
>> I believe Lee Luna suggested this but for the life of me, I can’t find the
>> reference!
>> 
>> 
>> 
>> Regards,
>> 
>> 
>> 
>> Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired)
>> 
>> Principal Scientist, the Children’s Hospital at Westmead (Retired)
>> 
>> Adjunct Fellow, School of Medicine, University of Western Sydney.
>> 
>> 
>> 
>> *From: *jayalakshmy p.s via Histonet <histonet at lists.utsouthwestern.edu>
>> *Sent: *Friday, 10 November 2023 3:43 AM
>> *To: *histonet at lists.utsouthwestern.edu
>> *Subject: *[Histonet] Faded H&E tissue section
>> 
>> 
>> 
>> Hello,
>> I would like to know how to effectively restain a faded H&E tissue section.
>> The color becomes dull when re stained. Somebody please advise.
>> Prof. Jayalakshmy. P S
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>> 
>> 
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