[Histonet] Faded H&E tissue section

jayalakshmy p.s psjayalakshmy at gmail.com
Thu Nov 9 20:11:19 CST 2023


Thanks, I'll check it out.

On Fri, Nov 10, 2023, 6:58 AM Tony Henwood <afhenwood at outlook.com> wrote:

> You could treat the decoverslipped section in 1% periodic acid (same as
> used in the PAS technique)  for 30 minutes prior to H&E staining. This
> might improve the H&E staining.
>
>
>
> I believe Lee Luna suggested this but for the life of me, I can’t find the
> reference!
>
>
>
> Regards,
>
>
>
> Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired)
>
> Principal Scientist, the Children’s Hospital at Westmead (Retired)
>
> Adjunct Fellow, School of Medicine, University of Western Sydney.
>
>
>
> *From: *jayalakshmy p.s via Histonet <histonet at lists.utsouthwestern.edu>
> *Sent: *Friday, 10 November 2023 3:43 AM
> *To: *histonet at lists.utsouthwestern.edu
> *Subject: *[Histonet] Faded H&E tissue section
>
>
>
> Hello,
> I would like to know how to effectively restain a faded H&E tissue section.
> The color becomes dull when re stained. Somebody please advise.
> Prof. Jayalakshmy. P S
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